NPP > JOURNALS > FGR > Vol. 57 (2010)
Abstract
Here we report the construction and application of a range of expression plasmids designed to facilitate live-cell imaging of F-actin dynamics in filamentous fungi simultaneously with other, preferably GFP-tagged fusion proteins. Pros and cons of the use of three different red fluorescent proteins (RFPs), two different promoters and three different selection markers are addressed.
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Recommended Citation
Lichius, A.,
and
N.D. Read
(2010)
"A versatile set of Lifeact-RFP expression plasmids for live-cell imaging of F-actin in filamentous fungi,"
Fungal Genetics Reports:
Vol. 57, Article 4.
https://doi.org/10.4148/1941-4765.1070
