NPP > JOURNALS > FGR > Vol. 64 (2020)
Abstract
Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of Neurospora crassa and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: N. sitophila. Specifically, we show that deletion of N. sitophila mus-51 increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the N. sitophila mus-51∆ strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC, Kansas State University, Manhattan, KS).
Recommended Citation
Rhoades, N. A.,
E.K. Webber,
and
T.M. Hammond
(2020)
"A Nonhomologous End-Joining Mutant for Neurospora sitophila Research,"
Fungal Genetics Reports:
Vol. 64, Article 1.
https://doi.org/10.4148/1941-4765.2172
