Predicting the Identities of su(met-2) and met-3 in Neurospora crassa by Genome Resequencing

Authors

Kevin McCluskey, Fungal Genetics Stock Center, Kansas State University, Manhattan KSFollow
Daren Brown, USDA ARS NCAURFollow
Erin Bredeweg, Pacific Northwest National LaboratoryFollow
Scott E. Baker, Pacific Northwest National LaboratoryFollow

Abstract

A significant number of classical genetic Neurospora crassa biochemical mutants remain anonymous, unassociated with a physical genome locus. By utilizing short read next-generation sequencing methods, it is possible to sequence the genomes of mutant strains rapidly and economically for the purpose of identifying genes associated with mutant phenotypes. We have taken this approach to connect genes and mutations to “methionineless” phenotypes in N. crassa.

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Recommended Citation

McCluskey, K., D. Brown, E. Bredeweg, and S.E. Baker (2024) "Predicting the Identities of su(met-2) and met-3 in Neurospora crassa by Genome Resequencing," Fungal Genetics Reports: Vol. 67, Article 3. https://doi.org/10.4148/1941-4765.2183