Swine day, 1981; Kansas Agricultural Experiment Station contribution; no. 82-128-S; Report of progress (Kansas State University. Agricultural Experiment Station and Cooperative Extension Service); 406; Swine; Diarrhea; K88 E. coli


The efficiency of the standard agglutination test, Y-1 mouse adrenal cell test, and infant-mouse gastric test was compared with a recently developed enzyme-linked immunosorbent assay (ELISA) for detecting the K88 pilus antigen and enterotoxin-producing E. coli. Isolates from clinical samples were identified on suspensions of bacteria. The sensitivity of the assay was in the nanogram-per-ml range as determined by measuring purified pili.; Swine Day, Manhattan, KS, November 12, 1981

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