swine, vaccine, adjuvant, classical swine fever
Emulsion-based adjuvants are commonly used in animal vaccine formulations for several reasons including affordability, stability, and efficacy in inducing disease-protecting immune responses. Here we report a novel, cost-effective, stable, self-emulsifying adjuvant (SEA1) that is prepared by a simple low shear process or low-energy mixing without the use of expensive and complex proprietary equipment. Characterization of the SEA1 adjuvant showed good stability at different temperatures (4°C, 20°C, and 37°C) after one month of storage. Minimal changes in droplet size distribution, polydispersity index, Zeta potential and pH in 1-month-old SEA1 preparations were observed when compared with a fresh SEA1 preparation. SEA1 emulsion-based experimental vaccine preparations effectively stimulated humoral immunoglobulin (IgG) responses in mice and swine and were comparable to commercially available adjuvants Montanide ISA 201 and 206.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Madera, Rachel; Burakova, Yulia; Wang, Lihua; and Shi, Jishu
"Development of a Self-Emulsifying Adjuvant for Use in Swine Vaccines,"
Kansas Agricultural Experiment Station Research Reports: