Escherichia coli, Salmonella, probiotics, Probicon L28, BioPlus 2B, market pigs, pre-harvest interventions


Pigs are hosts forSalmonellaand Shiga toxin-producingEscherichia coli(STEC) and these pathogens can commonly be isolated from the pig farm environment. Pigs can carry pathogens to the abattoir and contaminate pork products, posing a risk to public health. Identifying an intervention that effectively reduces pathogens in commercial pigs before harvest is imperative. Due to the need for effective pre-harvest interventions in the pig industry, the objective of this study was to investigate BIOPLUS 2B (Bacillus licheniformisandBacillus subtilis) and Probicon L28 (Lactobacillus salivariusL28) as pre-harvest interventions to reduceSalmonellaand STEC in commercial growing-finishing pigs. Two groups of pigs (group 1, N = 294; group 2, N = 356, initial body weight = 106.6 lb) were fed a standard corn-soybean meal (SBM) finishing diet according to the following treatments: Probicon L28 supplementation through water lines at 1.0 × 106 CFU/head/day (Probicon); BIOPLUS 2B supplemented at 3.0 × 109 CFU/head/day (BIOPLUS 2B); and a control with no added probiotic (Control). With each group of pigs, 12 pens were used per treatment (N = 24 total), for a total of 36 pens per group (N = 72 pens total). Each group was sampled upon arrival/baseline, midway through the grow-finish phase/6 weeks post-placement, and prior to loadout/13 weeks post-placement to collect fecal samples (4 pigs/pen), boot covers (2/pen), and ropes (1/pen). Market pigs were followed to the abattoir and superficial inguinal lymph nodes (SILNs) were collected. All samples were analyzed for STEC (stx, eaegenes, and O157:H7, and O26, O111, O121 O45, O103, and O145 serogroups) andSalmonellausing the BAX System (real-time polymerase chain reaction). Overall,Salmonellaand O111 prevalence was very low for all sample types, andEscherichia coliO157:H7 was not detected in any samples throughout the study. When compared to the control, there was no evidence (P>0.05) that BIOPLUS 2B and Probicon L28 impacted the prevalence of STEC (stxandeaegenes) or serogroups O26, O121, O45, O103, and O145 in feces, boot covers, ropes, and SILNs of market pigs.


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